DNA barcoding of the genus Alburnoides Jeitteles, 1861 (Actinopterygii, Cyprinidae) from Anatolia, Turkey

The present study investigated the ability of DNA barcoding to reliably identify endemic freshwater species in Turkey, known as biodiversity hotspots. barcode region (652 bp) mitochondrial cytochrome c oxidase subunit I (COI) was used 153 individuals from 13 morphologically identified genus Alburnoides . Based on Kimura two-parameter (K2P) evolution model, average interspecific distance (0.0595) 31-fold higher than intraspecific (0.0019). There a clear-cut gap (0.0158–0.0187) between maximum ( A. tzanevi and velioglui ) minimum nearest-neighbour freyhofi kurui for Anatolian common genetic threshold 0.0158 sequence divergence defined delimitation. multiple delimitation methods (ABGD, ASAP, GMYC bPTP) revealed total 11 molecular operational taxonomic units (MOTUs) morphospecies. Neighbour-joining (NJ), Maximum Likelihood (ML) Bayesian Inference (BI) tree analysis indicated that all haplotypes were clustered into two major clades, which corresponded eleven clusters, with strong bootstrap support. Furthermore, specimens concurrence morpho-taxonomic status species, except coskuncelebii emineae differentiated, but showed overlap variation COI-based data other species. Overall, results is effective identification cataloguing Turkey.